Development and Customization of Protein-Free, Animal Component-Free Media for the Enhanced Expression of Recombinant Proteins in Chinese Hamster Ovary (CHO) Cells
نویسندگان
چکیده
The expression of recombinant proteins has increased in importance for both research and biopharmaceutical manufacturing applications. Chinese Hamster Ovary (CHO) cell lines are one of the most frequently used cell lines for the expression of recombinant proteins that require posttranslational modification to exhibit full biological function. CHO cells used for large-scale production of recombinant proteins are often grown as suspension cultures using traditional serum-supplemented medium. For biopharmaceutical manufacturers the use of serum-supplemented medium poses numerous difficulties. If possible, most therapeutic producers would prefer to eliminate all animal-derived components from their culture systems.
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